Human Pluripotent Stem Cell Array Kit

INTRODUCTION

Analyzing the expression profiles of stem cell markers is helpful for understanding the
differentiation mechanisms of human stem cells and developing disease treatments. The
Human Pluripotent Stem Cell Array is a rapid, sensitive, and economical tool to simultaneously
detect the relative levels of expression of 15 stem cell markers without performing numerous
individual immunoprecipitations and Western blots. Each antibody was carefully selected using
cellular extracts prepared from cell lines known to express the target protein.

PRINCIPLE OF THE ASSAY

Capture and control antibodies have been spotted in duplicate on nitrocellulose membranes.
Cellular extracts are diluted and incubated overnight with the Human Pluripotent Stem Cell
Array. The array is washed to remove unbound proteins, followed by incubation with a cocktail
of biotinylated detection antibodies. Streptavidin-HRP and chemiluminescent detection
reagents are applied, and a signal is produced at each capture spot corresponding to the
amount of protein bound. Refer to the Appendix for a list and coordinates of analytes and