Safety Data Sheet

Product Name: Recombinant Zebrafish VEGF 165

5. Fire Fighting Measures:

Reviewed on: 27 February 2012

   Suitable extinguishing agents: Dry chemical, carbon dioxide, water spray or alcohol-resistant foam. Do not extinguish fire unless flow can be stopped. Use water in flooding quantities as fog. Solid streams of water may be ineffective. Cool all affected contain ers with flooding quantities of water. Apply water from as far a distance as possible.

   Special hazards arising from the substance or mixture: Emits toxic fumes under fire conditions. Carbon oxides.

   Protective equipment: Wear self contained breathing apparatus and protective clothing to prevent contact with skin and eyes.

   Further information: Use water spray to cool unopened containers.

6. Accidental Release Measures:

      Personal precautions, protective equipment and emergency procedures: Wear respiratory protection. Avoid breathing vapors, mist or gas. Ensure adequate ventilation. Remove all sources of ignition. Evacuate all personnel to safe areas. Beware of vapors accu mulating to form explosive concentrations. Vapors can accumulate in low areas.

           Environmental precautions: Prevent further leakage or spillage is safe to do so. Do not let product enter drains. Discharge into the

environment must be avoided.

   Methods and materials for containment and cleaning up: Contain spillage, and then collect with an electrically protected vacuum

cleaner or by wet-brushing and place in container for disposal according to local regulations.

7. Handling and Storage:

   Precautions for safe handling:

Protective measures: Put on appropriate personal protective equipment (see Section 8). Do not get in eyes or on skin or clothing. Do

not breathe vapor or mist. Do not ingest. Use only with adequate ventilation. Wear appropriate respirator when ventilation is inadequate. Do not enter storage areas and confined spaces unless adequately ventilated. Keep in the original container or an approved alternative made from a compatible material, kept tightly closed when not in use. Store and use away from heat, sparks, open flame or any other ignition source. Use explosion-proof electrical (ventilating, lighting and material handling) equipment. Use only non-sparking tools. Take precautionary measures against electrostatic discharges. Empty containers retain product residue and can

be hazardous. Do not reuse container.

Advice on general occupational hygiene: Eating, drinking and smoking should be prohibited in areas where this material is handled,

stored and processed. Workers should wash hands and face before eating, drinking and smoking. Remove contaminated clothing and protective equipment before entering eating areas. See also Section 8 for additional information on hygiene measures.

   Conditions for safe storage: Store in a well-ventilated place. Keep cool. Store in accordance with local regulations. Store in a segregated

and approved area. Store in original container protected from direct sunlight in a dry, cool and well ventilated area, away from incompatible materials (see section 10) and food and drink. Store locked up. Eliminate all ignition sources. Separate from oxidizing materials. Keep container tightly closed and sealed until ready for use. Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabelled containers. Use appropriate containment to avoid environmental contamination.

8. Exposure Controls and Personal Protection:

   Control parameters:

Occupational exposure limits Product/ingredient name Exposure limit values

Acetonitrile (EH40/2005 WELs (United Kingdom (UK), 8/2007).

STEL: 102 mg/m³ 15 minute(s). STEL: 60 ppm 15 minute(s). TWA: 40 ppm 8 hour(s).

TWA: 68 mg/m³ 8 hour(s).

   Recommended monitoring procedures: If this product contains ingredients with exposure limits, personal, workplace atmosphere or

biological monitoring may be required to determine the effectiveness of the ventilation or other control measures and/or the necessity to use respiratory protective equipment. Reference should be made to European Standard EN 689 for methods for the assessment of exposure by inhalation to chemical agents and national guidance documents for methods for the determination of hazardous substances.

   Derived effect levels: No DELs available.

   Predicted effect concentrations: No PECs available.