Introduction

R&D Systems’ Human Phospho-Receptor Tyrosine Kinase (RTK) Array Kit (Catalog # ARY001B) is a screening tool designed to simultaneously detect the relative phosphorylation of 49 different RTKs. This kit eliminates the need for numerous immunoprecipitation (IP) and/or Western blot experiments. RTKs are captured by antibodies spotted on a nitrocellulose membrane. Levels of Phospho-RTK are then assessed using an HRP-conjugated pan phospho-tyrosine antibody followed by chemiluminescence detection.

• Product Insert (PDF, 950K)
• Troubleshooting Guide
• Assays for analytes represented in to the Human Phospho-Receptor Tyrosine Kinase Array Kit

General Assay Principle

Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell lysate samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any protein/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of cytokine bound. Chemiluminescence is detected in the same manner as a Western blot.

Kit Contents

• 4 Array Membranes
• 4-Well Multi-dish
• Array Buffers
• Lysis Buffer
• Wash Buffer
• Anti-Phospho-Tyrosine-HRP Detection Antibody
• Chemiluminescent Detection Reagents
• Transparency Overlay Template
• Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.