R&D Systems’ Human Phospho-Receptor Tyrosine Kinase (RTK) Array Kit (Catalog # ARY001B) is a screening tool designed to simultaneously detect the relative phosphorylation of 49 different RTKs. This kit eliminates the need for numerous immunoprecipitation (IP) and/or Western blot experiments. RTKs are captured by antibodies spotted on a nitrocellulose membrane. Levels of Phospho-RTK are then assessed using an HRP-conjugated pan phospho-tyrosine antibody followed by chemiluminescence detection.
- Product Insert (PDF, 950K)
- Troubleshooting Guide
- Assays for analytes represented in to the Human Phospho-Receptor Tyrosine Kinase Array Kit
|Simultaneously detect the relative phosphorylation of these RTKs in a single sample|
|Axl||EphB6||PDGF R alpha|
|DDR1||ErbB2||PDGF R beta|
|EGF R||FGF R1||ROR2|
|EphA1||FGF R2 alpha||Ryk|
|EphA2||FGF R3||SCF R/c-kit|
|EphA10||M-CSF R||VEGF R1/Flt-1|
|EphB2||MSP R/Ron||VEGF R3/Flt-4|
General Assay Principle
Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell lysate samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any protein/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of cytokine bound. Chemiluminescence is detected in the same manner as a Western blot.
- 4 Array Membranes
- 4-Well Multi-dish
- Array Buffers
- Lysis Buffer
- Wash Buffer
- Anti-Phospho-Tyrosine-HRP Detection Antibody
- Chemiluminescent Detection Reagents
- Transparency Overlay Template
- Detailed Protocol
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.