To reliably induce epithelial to mesenchymal transition (EMT) in vitro.
- Induces EMT in only 5 days
- Is compatible with multiple cell types
- Is a defined formulation to provide consistent EMT induction
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Why Induce EMT in vitro with a Defined Media Supplement?
Designed to study EMT in vitro, this media supplement contains a cocktail of EMT-inducing recombinant proteins and neutralizing antibodies for the straightforward induction of EMT in a variety of cell types.
Media Supplement Components
This defined media supplement contains the following premium quality proteins and high performance neutralizing antibodies to induce EMT: See Details
Induction of Epithelial to Mesenchymal Transition(EMT) in the A549 human lung carcinoma cell line. The A549 human lung carcinoma cell line was cultured without (A, B); or with (C, D) the StemXVivo EMT Inducing Media Supplement (Catalog #CCM017) for five days. (A) Control cells exhibit typical epithelial morphology compared to the spindle-shaped, mesenchymal morphology observed in cells following EMT induction (C). EMT status was also assessed by dual immunofluorescence. (B) Untreated cells express the epithelial marker E-Cadherin (red) and exhibit little labeling of the mesenchymal marker Fibronectin (green). (D) In contrast, EMT induction results in decreased E-Cadherin expression (red) and an increase in Fibronectin labeling (green). E-Cadherin was detected in cells using a NorthernLights™ (NL) 577-conjugated Goat Anti-Human E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # NL648R; red). Fibronectin was detected using a Mouse Anti-Human Fibronectin Monoclonal Antibody (Catalog # MAB1918), followed by a NL493-conjugated Donkey Anti-Mouse IgG Secondary Antibody (Catalog # NL009; green). The nuclei were counterstained with DAPI (blue).
Upregulation of the Mesenchymal Markers, Snail and Vimentin, in EMT-induced Cells. A549 human lung carcinoma and MCF 10A human breast epithelial cells were either untreated or cultured with the StemXVivo™ EMT Inducing Media Supplement (Catalog # CCM017) for five days. The cells were analyzed for an epithelial to mesenchymal transition (EMT) by simultaneously staining with the antibodies contained in the Human EMT 3-Color Immunocytochemistry Kit (Catalog # SC026): NorthernLights™ (NL)637-conjugated Goat Anti-Human E-Cadherin (pseudocolored white), NL557-conjugated Goat Anti-Human Snail (red), and NL493-conjugated Rat Anti-Human Vimentin (green). Cells cultured with the EMT Inducing Media Supplement showed downregulation of the epithelial marker, E-Cadherin, and concurrent upregulation of the mesenchymal markers, Snail and Vimentin, compared to control cells.